Device

Part:BBa_K1913003:Design

Designed by: Lisa Röttjers   Group: iGEM16_Wageningen_UR   (2016-09-22)


chiB device regulated by pBAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1423
    Illegal BamHI site found at 2242
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1348
    Illegal NgoMIV site found at 1787
    Illegal NgoMIV site found at 1793
    Illegal NgoMIV site found at 1886
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1994


Design Notes

This is a composite part for expression of the chiB protein coding region from BBa_K1913001.


Source

Original chiB sequence originates from GQ855218.

References